The matrix (inside mito) has a negative charge compared to the intermembrane space (outside of mito but still inside) because positively charged protons are constantly pumped from the matrix to the intermembrane space. A cell oxidizes an organic molecule producing 3 NADH molecules and 3 FADH2 molecules..
The gene p53, also called the guardian of the genome is located on the 17th chromosome in human genomes.
It was given this alternative name because it has a role in response to damage of DNA molecule and in preventing cancer.
The protein product of this gene stops the replication of damaged DNA, activates proteins that repair the DNA damage and if the reparation proteins fail to fix the damage it activates the process of apoptosis or so-called cell death in order to prevent the damaged cell to proliferate and potentially develop into a tumor.
Only eukaryotes contain upper three but all cells contain "Contractile Vacuoles"
In short, Your Answer would be Option D
Hope this helps!