Question

Consider the DNA sequence. 5' C C G T A A C A G T C G A C G A G C A C A T A G C T T C A G C A T A C A G C A G A T A G 3' 3' G G C A T T G T C A G C T G C T C G T G T A T C G A A G T C G T A T G T C G T C T A T C 5' Select the pair of primers that are appropriate for amplifying the sequence of DNA during a polymerase chain reaction (PCR).

Answer 1

Answer:

Primers should be:

3 'ACGTATGTCGCTTAGT 5'

5 'ACGTTCCAGTCAATGA 3'

Explanation:

DNA replication occurs with the need for the presence of primers that are a small sequence of nitrogenous bases that will allow the initiation of replication.

Primers are extremely important in PCR and are represented as small pieces of single-stranded DNA that specify the region of DNA that should be replicated during PCR. These primers attach to the single strand of DNA by pairing the nitrogenous bases allowing the DNA polymerase to recognize where replication begins.

Regarding the question above, the primers should be:

3 'ACGTATGTCGCTTAGT 5'

5 'ACGTTCCAGTCAATGA 3'