Answer:
b. Forward or reverse primers
Explanation:
Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.
It is called ACCOMMODATION. Accommodation refers to the mechanism by which the eye adjusts his optical power in order to focus properly on an object as the distance of the object varies. To achieve this, the shape of the lens has to be adjusted appropriately.
Herbaceous mano it has vascular bundles arranged in a ring around the pit and herbaceous dicot stems has a vascular cambium between xylem and phloem
I believe it is A. Nerve. I am pretty positive I am right.
1. 32 c
2. 32 f
3. 212 c
4. 212 f
5.100 f
Hope this helps
