Adenosine monophosphate (AMP) is a regulatory molecule in metabolic processes such as glycolysis and gluconeogenesis. For example, it stimulates the glycolytic enzyme phosphofructokinase, and therefore ATP production, and it inhibits the gluconeogenic enzyme fructose 1,6-bisphosphatase. Adenylate kinase catalyzes the reversible reaction shown here:
2ADP --> ATP + AMP
During periods of intense activity, when glycolysis is used in the generation of ATP, the reaction lies to the right, decreasing [ADP], generating ATP, and accumulating AMP. However, [ATP] is usually much greater than [ADP], and [ADP] is greater than [AMP].
Determine [AMP] when 3% of the ATP in a hypothetical cell is hydrolyzed to ADP.
<span>In this cell, the initial concentration of ATP is 265 ?M, and the total adenine nucleotide concentration (the concentration of ATP, ADP, and AMP) is 368 ?M. The equilibrium constant K is 0.82</span>
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