52 chromosomes in each somatic cell. In the sex cells, there would be 26, because they contain one copy of each chromosome (haploid) while the somatic cells have two (diploid). Mitosis is the division of somatic cells, which results in two identical daughter cells each with 2 copies of each chromosome.
The substance that must come through the cell membrane for the cell to live is nutrients. Cells would die if they did not get nutrients. Nutrients are what keeps the cell running.