Answer:
if im not mistaken the answer should be the first one
<span>The fluid filled region between the plasma and nucleus is called cytoplasm. This region is a gel like substance and it contain all of the cell structure which is about water and the area that most cell function occur.</span>
I'm assuming you mean a specific area, the scientific term being a neoplasm.
This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
