How is the p+ bacteria culture treated differently from the p- bacteria culture? (a culture is an isolated population of cells.)

Question

4 years ago

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what is the purpose of the p- bacteria culture?

1 answer:

Alenkasestr [34] · Answer 1 · 2022-01-30T20:30:25+03:00

Answer:

The P+ culture contains inserted DNA plasmid while P- culture does not contain DNA plasmid.
The purpose of P- bacteria culture is to take it as plasmid control.

Explanation:

There are two groups of bacteria using in the lab; one is control group and second is treatment group. Control group is denoted as P- group while treatment group is denoted as P+ group.
To differentiate between these two group we used the culture medium called Luria Broth with addition of an antibiotic ampicillin and the sugar arabinose.
The control group (P-) does not have plasmid stay same throughout the process because of absent of sugar arabinose.
The treatment group (P+) having plasmid show more growth due to arabinose.