Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid. The most common Type II enzymes are those like HhaI (NEB #R0139), HindIII (NEB #R0104), and NotI (NEB #R0189)
Multi cellular organisms are composed of many cells whereas unicellular organisms are composed of single cell. Multicellularity is indeed a progressive attribute of evolution where cells form tissue which forms organ and then organ system and finally an organism. Both multi cellular and unicellular organisms has advantages and disadvantages of their own. One of the main disadvantage of multi cellular organisms is that due to such a complex composition and functioning they require a large amount of energy for their maintenance and survival. Different organs and system require a huge amount of energy when it comes to comparison with unicellular organisms. A large amount of energy is also wasted in all these life processes. Though multi cellular organisms can survive in a variety of environmental conditions but then also their survival is difficult than any unicellular organism.
