Nothing I really don’t think there is an answer to that
Answer:
D) fertilization
Explanation:
Fertilization is the name given to the fusion of male and female gametes. By themselves, female egg cells -- called ova -- and male sperm cells cannot grow and divide. However, once a sperm cell breaks through the egg cell membrane, the genetic material combines and the new organism begins to grow and develop as a living thing.
That represents geographical isolation.
Biological isolation is present where members of species are not able to produce fertile offspring due to the genetic incompatibility.
Temporal isolation includes differences in the seasons of mating. One species mates in season x and another in season y, means they will not be able to interbreed.
Anatomical isolation means there are differences in the physical structures of the species members that inhabit their ability to mate.
A good expression vector for protein expression in coli should have features like an origin of replication, a selectable marker, a promotor, an operator, and a ribosome binding site.
A Ribosome Binding Site (RBS) is a part or segment of the upstream part of a messenger RNA (mRNA) molecule which binds the ribosome to position the message correctly for the initiation of translation.
Selectable marker genes are conditionally dominant genes that confer an ability to grow in the presence of applied selective agents that are normally toxic to cells or inhibitory to growth, such as antibiotics and herbicides.
Learn more about expression here
brainly.com/question/14972953
#SPJ4