An action potential is caused by either threshold or suprathreshold stimuli upon a neuron. It consists of four phases; hypopolarization, depolarization, overshoot, and repolarization. An action potential propagates along the cell membrane of an axon until it reaches the terminal button.
Answer:
The correct answer is -
II: lyse cells of interest, precipitate DNA
V. Digest (cut) genomic DNA with restriction enzyme
I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!
IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.
III Introduce constructs via transformation into competent E. coli cells.
Explanation:
The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.
The genomic library construction involved some major steps that are:
- Extract and purify DNA - lysing and separating the desired DNA of interest.
- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes
- cut phage (chromosome) with restriction enzyme
- Insert the fragments of DNA into vectors
- introduce these recombinant a host bacterium by transformation, creating a DNA library.
Answer:
DNA ligase
Explanation:
After Okazaki fragments (sequences complementary to the lagging strand of DNA) are produced, they must be joined together to produce a continuous strand. The Okazaki fragments are then joined up by DNA ligase, which catalyzes the formation of a phosphodiester bond between the neighboring nucleotides.
Answer:
Search the the periodic table for more info
Explanation:
Metals are, well, metals. Examples are Iron, Copper, and Aluminum.
Non Metals are simple; they are not metals
Metalloids are complex to define, so I will let the Oxford Dictionary explain for me;
an element whose properties are intermediate between those of metals and solid nonmetals or semiconductors.
(the oxford dictionary)