Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Effects on biodiversity at different scales, geographical regions, and environments. Mostly humidity and temperature.
Explanation:
Isopod distribution is tightly connected to available habitats and habitat features at a fine spatial scale, even though different species may exhibit a variety of responses
In plants, transpiration is a process which is inevitable but potentially harmful, because it can cause loss of water. Loss of water can lead to wilting and eventual death of the plant. Even a little stress can interfere with the plant's growth process.
But, at the same time, transpiration is necessary because of a number of reasons - the transpiration stream aids the processes of mineral absorption, water absorption, energy exchange, helps provide the plant with optimum turgidity, it helps the exchange of gases and so on.
Answer:
When a volcano erupts particles of rock and ash are released into the atmosphere after this water droplets form around?
When a volcano erupts, particles of rock and ash are released into the atmosphere. After this, water droplets form around the rock and ash particles and fall to Earth as rain. The rainwater helps plants grow. Which scenario describes an interaction between two of Earth's spheres?
Explanation:
