Answer:
A. RNA primase: It is a DNA dependent RNA polymerase which synthesize short sequences of RNA which serve as primers. They provide 3-OH group as a initiating point to the DNA polymerase in DNA replication.
Hence, it can be correctly matched with 3. synthesizes RNA primer at the initiation point
B. Ligase: DNA ligase is also called as molecular glue as it can join two DNA fragments by forming phospo-di-ester bond between them. In DNA replication, it is used to join Okazhaki fragments formed in the lagging strand.
Hence, it can be correctly matched with 5. joins the Okazaki fragments together
.
C. Helicase: these are ATP driven enzyme which unwinds the DNA and help in forming the replication fork. They separate the two strands of the DNA so that each strand can be copied.
Hence, it can be correctly matched with 1. unwinds the DNA molecule.
D. Polymerase: DNA polymerase is DNA-dependent-DNA polymerase which catalyses the synthesis of new DNA strand based on the complementarity of the template strand.
Hence, it can be correctly matched with 2. synthesizes the complementary daughter strands.
E. Topoisomerase: DNA topoisomerase are the enzymes which regulate the super-coiling of the DNA by catalyzing the unwinding and winding of the DNA strands.
In replication, they travel ahead of DNA polymerase and relieve the tension produced by unwinding the DNA.
Hence, it can be correctly matched with 4. creates nicks ahead of the replication fork to relieve tension from unwinding
