Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
Answer:All living organisms are composed of cells, from just one to many trillions, whose details usually are visible only through a microscope.
Explanation:
Answer:
independent variable is number of apples
dependent variable is grades
Answer:
A.
Explanation:
A parasite does not feed it's host; in fact, it takes food energy/ food from it's host.
Answer:
What Are the Benefits and Risks of the Procedure? Dr. ...
How Much of the Tumor Will Be Removed? ...
What Will Be the Next Steps After Surgery? ...
What Clinical Trials Are Going on That Might Be a Good Fit? ...
What Should I Expect Down the Line Regarding My Treatment and Health?
Explanation: