Answer:
It is necessary because of the base pairing between the desired gene and plasmid
Explanation:
Restriction enzymes are enzymes that recognize the specific DNA sequence-restriction site, double-stranded cut DNA and produce ends with a single-stranded overhang. The overhangs of the plasmid can stick together with overhangs of gene of interest by complementary base pairing. Joining of ovarhangs is done by the enzyme DNA ligase.
Explanation:
with brown bodies and red eyes (BbEe X BbEe) should yield offspring with nine possible genotypes (BBEE, ... red eyes, brown body with brown eyes, black body with red eyes, and black body with brown eyes ...
Is biotic?
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