Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
Answer: I think it's a don't blame me if it's wrong though
Explanation:
Answer:
If this trend continues, the following week will be cooler, and a large amount of rain will fall.
Explanation:
Patterns and trends can often be found in data sets. During the week that Cho recorded the weather, the temperatures consistently dropped by one to four degrees each day. At the end of the week, the amount of precipitation increased daily.
The configuration is 1s2 . Hope I answered and helped out! :)
That's because the solubility
- Temperature is directly proportional to solubility
Higher the solubility higher the temperature
Lower the temperature lower the solubility
So
Less temperature makes enzymes work faster
