Identify the plasmid that must contain the CD3γ core promoter sequence but the fewest or no negative regulatory sequences. Based
on the data in Figure 2, describe the most likely cause of the variation in luciferase activity among the cells that contain plasmids pCD3γ-419 , pCD3γ-309 , pCD3γ-239 , and pCD3γ-199 . Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3γ-59 and cells containing plasmid pCD3γ-149 . Round to the nearest whole number.
The plasmid that must contain the CD3y core promoter sequence is PCD3γ-15, and the most likely cause in variation is due to the differing regulatory sequences in the cell.
