Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
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Explanation:
The development of electronic delivery systems advancements in the music industry has preserved resources and reduced waste.
Option C
<h3><u>Explanation:</u></h3>
Music industry is the industry that deals with the production of new music that are soothing to hear, recording them, spreading them in public and so on. This industry is an age old industry which used to record music in different ways in past. Most of them were not that efficient to capture the individual tunes of the music and moreover they used the resources very inefficiently.
With the advent of modern day electronic delivery systems, the mics came into market, as well as different electronic media like pendrive, CD's, tape recorders, and even internet through which music can be spread among public very easily.
