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mariarad [96]
3 years ago
9

PLEASEEE ANSWER IN NEXT 5 MIN Why do the cells need time to recover after the heat shock?

Biology
1 answer:
Margarita [4]3 years ago
5 0

Answer:

Following heat shock or electroporation, transformed cells are cultured in antibiotic-free liquid medium for a short period to allow expression of antibiotic resistance gene(s) from the acquired plasmid to begin (Figure 5). This step improves cell viability and cloning efficiency.

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