3 years ago

PLEASEEE ANSWER IN NEXT 5 MIN Why do the cells need time to recover after the heat shock?

1 answer:

5 0

Answer:

Following heat shock or electroporation, transformed cells are cultured in antibiotic-free liquid medium for a short period to allow expression of antibiotic resistance gene(s) from the acquired plasmid to begin (Figure 5). This step improves cell viability and cloning efficiency.

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