Answer:
Cas9-sgRNA cannot align with targeted DNA sequence
Explanation:
Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.
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Benign because benign tumors don't grow into nearby tissues or spread to distance areas
Water inside of the cell will flow outwards, and the cell will shrink.