Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
You must pack food that has high concentrations of fat and protein. Food like granola bars and canned meat are a good source of energy and heat. This is because the body breaks this food down easily converting it into usable energy. Though some people would suggest that you pack food that are high in carbohydrates, fats and proteins are long-term sources of energy that you would need for your hiking trip.
The four factors that affect evolution are mutations, natural selection, genetic drift and the gene flow, In the case, if the ground finches that Grants studied, we can observe the working of the natural selection. Due to change in the conditions in the environment, the large beak was an advantageous trait that was favored by the natural selection. The result of this process was an increase in numbers of large beaked finches and decrease in small-beaked finches compared to the initial numbers in the population.
blood type B could have a genotype of either BB or BO.
1.44 solar masses :) you are welcome
