The role of the Golgi body or Golgi apparatus is to package the proteins in vesicles and target it to their respective place of function. Example, the receptor proteins that are synthesised in the Endoplasmic Reticulum will be sent to the Golgi body for its packaging into the vesicles that bind and fuse with the cell membrane. The targeting is done by the special set of markers present on the secreted vesicle. All the eukaryotic cells have a Golgi apparatus. When chemical treatment is done to stop its functioning, the cell stops functioning and eventually dies due to the hampered targeting of proteins to the various organelle. On the other hand, prokaryotic cells lack Golgi body as the cell size is small enough to allow for the synthesised protein to move by diffusion to its target. Hence no effect will be seen when prokaryotic cells are treated with that chemical.
The pKa represents the pH of the medium at which the zwitterionic amino acid assumes most stable ionic form due to structural stabilization. As the pKa is dependent upon the environmental factors of the solution around the amino acids, a change in their structure and localization can cause change in the pKa of the protein. Thus, the answers can be found as below:
Part A: Decrease (As the lysine is basic in nature, it will tend to stabilize the electrostatic interaction and weak interactions between the acidic amino acids and hydrogen bonds in the viscinity, thus lowering the pH and hence pKa of the protein)
Part B: Increase (As the carboxyl group is acidic in nature, removal of it will tend to increase the pKa since the basic amino acids will tend to accumulate more negative charge in their viscinity)
Part C: Increase (As glutamic acid is an acidic amino acid, its shift from outside to a non-polar site will prevents its ionization and hence the pKa will tend to shift from slightly acidic to slightly basic, hence increase)
The answer is A. An organism living at the bottom of the ocean is used to a high pressure so they cannot study them as the lower pressure kills the organisms as they reach the surface.
4.) mumps or Mononucleosis