1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
lyudmila [28]
3 years ago
11

After running your purified DHFR fusion protein+Laemmli 40uL sample, along with a protein ladder you transfer protein on the gel

to a nitrocellulose filter for Western Blot analysis.
a. Which of the following primary antibodies could you use to detect your fusion protein based on the fusion protein identified and process described above: Anti-Myc, Anti-FLAG, Anti-GST, Anti-His, Anti-DHFR, Anti-Tubulin? List all that apply
Biology
2 answers:
Amanda [17]3 years ago
8 0

Answer:

Anti-his and Anti-DHFR antibodies can be used to detect the fusion as the primary antibodies in the process.

Explanation:

Western blot is often used in research to separate and identify proteins. Two major processes are involved in Western blot, namely, SDS-polyacrylamide gel electrophoresis and protein blotting and testing.

In the process above, the DHFR fusion protein was purified and run in the SDS-PAGE ( sds- page separates protein based on molecular weight) for the western blotting. This DHFR protein was fused with histadine tag which is why the antibody (anti-his) can be used as a primary antibody (Primary antibodies directly bind to the protein of interest). Anti DHFR can also be used as a primary antibody.

Vinil7 [7]3 years ago
4 0

Answer:

Explanation:

The DHFR protein was fused with His tag. The DHFR fusion protein was purified and run in the SDS-PAGE for the western blotting. After the completion of gel run, the protein was transferred into the nitrocellulose membrane.

a. For the detection of fusion protein two types of primary antibody can be used. These are Anti-his and Anti-DHFR.

Anti-his antibody can be used as the protein was tagged with histidine, and anti-DHFR antibody can also be used.

You might be interested in
Which organelle brakes down sugar molecule that supply energy to the cell
hjlf

The Mitochrodria helps break down sugar and turns it into energy

8 0
3 years ago
Put this in your own words. 12 points on the line!!!!!!
Solnce55 [7]

Answer:

Global warming is a change in the earth's climate which makes it heat up. While the greenhouse effect is a natural process that always is happening because of sunlight and the atmosphere.

I hope this is easy enough.

3 0
3 years ago
The reproductive organ that produces gametes is called a
svp [43]
Gonads are sex organs that produce gametes. Male gonads are the testes, which produce sperm and male sex hormones. Female gonads are the ovaries, which produce eggs (ova) and female sex hormones.
5 0
2 years ago
Gregor Mendel preformed his experiments by cross-pollinating various types of pea plants. True or False​
andrew-mc [135]

Answer:

Yes

Explanation:

5 0
2 years ago
Allows the cell membrane to engulf large molecules.<br> Diffusion<br> Osmosis<br> Active Transport
Zielflug [23.3K]
Active transport, specifically phagocytosis
8 0
2 years ago
Other questions:
  • 1. Diabetes is a disease where the body is unable to produce enough insulin in the pancreas to help lower the levels of blood gl
    14·1 answer
  • What is the next step in the scientific method, following stating a question?
    9·1 answer
  • Why is homeostasis important to the human body?
    12·1 answer
  • 9. What are groups of similar cells that work together to perform a specific function called? (1 point)
    10·2 answers
  • Carbon credits are used to a. rehabilitate tropical rainforests b. reduce the emission of volatile organic compounds (VOC's) c.
    15·2 answers
  • Scientists have studied a fruit fly, Drosophila, to better understand early embryonic development and have discovered that the c
    9·1 answer
  • Describe at least two costs and two benefits of wind farms. !!!! ASAP plzz
    7·1 answer
  • The apoplast in plant tissues consists of ________.
    6·1 answer
  • Most archaeologists believe that the __________ was one of the earliest centers of plant and animal domestication.
    8·1 answer
  • Select all that apply.
    8·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!