I think they are all biotic factors..hmm if not take out whichever sounds the most not fitting.
Answer:
■Gene sequences would be used to make Probes for both the Southern and Northern blots.
■The probes will be used to view the presence of each gene with the use of isolated genomic DNA obtained from the isolated bacterium
■Each probes hybridized to the genome shows the pathway is isolated and point of the genes were involved in the substrate catabolism
■The carbon source in the isolate is derived from the substrate inducing the catabolic pathway as RNA determine transcripts present
■Probes hybridizing to the same sequences would be used to determine the gene activity for the pathway as seen in the southern one
■since all the genes present in the genome couldn't be identified, the northern would be important to work on
■Catabolic pathway is determined by the same genes. Hence, the need for gene/transcript probes to hybridize to the transcriptome.
Answer:
I'm pretty sure it is B, because of natural selection
it gives off protons because it's in a basic solution (pH>7)
