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Svetllana [295]
3 years ago
10

Why is it necessary to utilize probes for labeling particular dna sequences? how is this process accomplished?

Biology
1 answer:
Lana71 [14]3 years ago
7 0
importance of a probe
in hybridization process, molecular biologist label a short fragment of DNA or RNA, usually 10-1000 bases long tagged with a radioactive or fluorescent molecules. this fragment is usually referred to as hybridization probe.probe  important both in southern and northern blotting processed because it is  used to identify a certain sequence of DNA or RNA in a pool several sequences that have been obtained using nuclease.

process of labeling DNA sequence with a probe
after the DNA strand has been cut into several fragments using enzyme DNA nuclease, the fragments are separated using gel electrophoresis and then denatured using hcl to obtain strands. the single strand are then transferred to nylon membrane that has positive charge. the next step is where probes come in. to detect the DNA sequence of interest the nylon membrane is treated with solution containing tagged probes. since the probes have complementary sequence to the target sequence, it bind to that DNA sequence and detected later using X-ray film.     
  


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Assume that producers in an ecosystem have 1,000,000 kilocalories of energy. How much energy is available to primary
castortr0y [4]

Answer:

1. 100,000 Kilocalories

2. Producers

Explanation:

1.

In a food chain, energy is being transferred when one organism in a particular trophic level feeds on another organism in a lower trophic level. The lowest trophic level contains the PRODUCERS, which are organisms that start the food chain. However, as each organism undergo its metabolic activities, most of the available energy (90%) is lost as heat. Only about 10% of energy is available to the organism in the next trophic level. In a nutshell, the higher the organism in the trophic level, the lower the amount of available energy.

In this case where it is assumed that that producers in an ecosystem have 1,000,000 kilocalories of energy, most of this energy (about 90%) will be lost as heat due to metabolic activities carried out by the plant. 10% of the energy contained in the plant will be transferred to Primary consumers in the next trophic level when they feed. Hence, 10/100 × 1,000,000 = 100,000 Kilocalories of energy will be available to primary consumers.

2.

The total biomass of an organism in each trophic level decreases going up. This is due to the lesser energy transferred from one trophic level to the next. As the trophic level increases, lower energy is available to convert into biomass. Hence, the higher the trophic level, the lower the biomass. If this is the case, the PRODUCERS at the first trophic level will contain the greatest biomass which will decrease as the food chain progresses upwards.

3 0
3 years ago
C6H12O6 + O2 --> CO2 + H2O + ATP
stiks02 [169]

There are, 7 Carbon atoms, 10 Oxygen atoms, 12 Hydrogen atoms in the reactants and 1 Carbon atom, 3 Oxygen atoms, 2 Hydrogen atoms in the products.

<h3>How to count the number of atoms in reactants and products?</h3>

  • Write down the chemical formula that is:
  • C_{6} H_{12} O_{6} + O_{2} → CO_{2} + H_{2} O + ATP

  • List all the atoms present in the equation:

C

H

O

  • Count the number of atoms of the element of each molecule in the reactant.

C=6

O=8

H=12

  • Count the number of atoms of the element of each molecule in the Product.

C=1

O=8

H=2

Hence there are,  6 Carbon atoms, 8 Oxygen atoms, 12 Hydrogen atoms in the reactants and 1 Carbon atom, 3 Oxygen atoms, 2 Hydrogen atoms in the products.

Refer the following link to know more about Atoms in equations:

brainly.com/question/7181548

#SPJ1

5 0
2 years ago
What is the genotype ratio for the offspring in this Punnett Square?<br><br> help
Gnoma [55]

Answer:

1:2:1

Explanation:

When we say genotype, we are talking about the different type of allele combinations. Phenotype is when you are looking at dominant recessive ratios.

7 0
3 years ago
what hormone secreted by the duodenum inhibits secretion of gastric juices and stimulates the release of insulin?
Sveta_85 [38]

Answer:

Gastric Inhibitory Peptide

Explanation:

3 0
3 years ago
WILL GIVE BRAINLIEST IF YOU ARE CORRECT!!!
Pachacha [2.7K]

Answer:

A. Fewer Nesting Sites.

Explanation:

if birds are spreading a disease to the trees that kills them off then that would lead to birds having less places for shelter, since that's the main thing they use trees for, unless were talking specifically about fruit and nut producing trees, then it would not only be lost of nesting sites, but it would also eventually be a loss of a food source as well.

6 0
2 years ago
Read 2 more answers
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