1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Svetllana [295]
3 years ago
10

Why is it necessary to utilize probes for labeling particular dna sequences? how is this process accomplished?

Biology
1 answer:
Lana71 [14]3 years ago
7 0
importance of a probe
in hybridization process, molecular biologist label a short fragment of DNA or RNA, usually 10-1000 bases long tagged with a radioactive or fluorescent molecules. this fragment is usually referred to as hybridization probe.probe  important both in southern and northern blotting processed because it is  used to identify a certain sequence of DNA or RNA in a pool several sequences that have been obtained using nuclease.

process of labeling DNA sequence with a probe
after the DNA strand has been cut into several fragments using enzyme DNA nuclease, the fragments are separated using gel electrophoresis and then denatured using hcl to obtain strands. the single strand are then transferred to nylon membrane that has positive charge. the next step is where probes come in. to detect the DNA sequence of interest the nylon membrane is treated with solution containing tagged probes. since the probes have complementary sequence to the target sequence, it bind to that DNA sequence and detected later using X-ray film.     
  


You might be interested in
Based on the similarities and differences you identified earlier between T. Brucei, P. Falciparum, and T. Cruzi, do you predict
OverLord2011 [107]

Answer:

Yes,  P. Falciparum and T. Cruzi undergo similar antigenic variation because of repetitive genomes evolved by time.

Explanation:

Living (i.e., actively proliferating) repeats are dynamic elements which reshape their host genomes by generating rearrangements, creating and destroying genes, shuffling existing genes, and modulating patterns of expression. Dead repeats (i.e., those which are no longer able to proliferate) constitute a palaeontological record, which can be mined for clues about evolutionary events and impetus. The dynamic nature of repeats leads to a rapid evolutionary divergence that can be used in species identification and phylogenetic inference. Repeats can also provide passive markers for studying processes of mutation and selection.

The genomes of these protozoan parasites, like all eukaryotic genomes, have been colonized by diverse repetitive elements. Repetitive sequences can be artificially divided into two groups: interspersed repeats and tandemly repeated DNA. P. falciparum undergoes antigenic variation ans similar anitgenic variation is present in t. cruzi because of repetitive sequences resembling each other.

7 0
3 years ago
The fossils in layer 4 are
Feliz [49]

Answer:

The fossils in layer 4 is much older than the fossils in layer 1

7 0
4 years ago
What do plants with no vascular tissue not have ?
iVinArrow [24]
The main difference between vascular and nonvascular plants is that a vascular plant has vascular vessels to carry water and food to all the different parts of the plant.
6 0
3 years ago
Read 2 more answers
The nurse is teaching a new group of mental health aides. the nurse should teach the aides that setting limits is most important
liq [111]

The nurse is teaching a new group of mental health aides. The nurse should teach the aides that setting limits is most important for a manic client.

As the nurse is teaching a new group of mental health aides, so, she is talking about manic clients and mental health aides are the people who take care of patients with mental disorder either in house or any centers.

<span>Manic client have bipolar disorder in which there is many unusual shifts of mood.</span>

7 0
3 years ago
Which type<br> Of lava would most likely form lava tube caves: a'a or pahoehoe
MA_775_DIABLO [31]
It's from the Hawaiian language and means the words "shiny, glassy surface, and smooth". The type of lava you want to know is Pahoehoe lava. Lava tubes are usually floored with it.

Hope this helps! :-)
3 0
4 years ago
Other questions:
  • Are skin and/or bone an organ?
    6·1 answer
  • Goiters are often produced in the _____, a gland that plays a key role in metabolism by secreting the thyroxine and triiodothyro
    7·1 answer
  • What is different between a bony fish and a cartilagnious fish
    8·1 answer
  • How do the organs and systems of an animal facilitate the creature's carrying out each of the characteristics of life mentioned
    6·1 answer
  • Help me on this someone
    14·1 answer
  • Which climatic zone is always hot and humid?
    6·1 answer
  • What Monomer is made of base, a suger, and phosphate group?
    15·1 answer
  • in a greenhouse you can control the temperature, light intensity, and carbon dioxide level in order to make it the what factor i
    7·1 answer
  • I really need help with this question​
    13·1 answer
  • Which of these changes to fuel consumption would lessen human
    7·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!