1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Svetllana [295]
2 years ago
10

Why is it necessary to utilize probes for labeling particular dna sequences? how is this process accomplished?

Biology
1 answer:
Lana71 [14]2 years ago
7 0
importance of a probe
in hybridization process, molecular biologist label a short fragment of DNA or RNA, usually 10-1000 bases long tagged with a radioactive or fluorescent molecules. this fragment is usually referred to as hybridization probe.probe  important both in southern and northern blotting processed because it is  used to identify a certain sequence of DNA or RNA in a pool several sequences that have been obtained using nuclease.

process of labeling DNA sequence with a probe
after the DNA strand has been cut into several fragments using enzyme DNA nuclease, the fragments are separated using gel electrophoresis and then denatured using hcl to obtain strands. the single strand are then transferred to nylon membrane that has positive charge. the next step is where probes come in. to detect the DNA sequence of interest the nylon membrane is treated with solution containing tagged probes. since the probes have complementary sequence to the target sequence, it bind to that DNA sequence and detected later using X-ray film.     
  


You might be interested in
Non-living things, such as a car, often show characteristics similar to those of living organisms.
Alex

Answer:

Living and car both move, both release energy, both needs energy to move and both gets damages in accidents.

6 0
2 years ago
Which of these best describes extinction events?
SpyIntel [72]

Answer: D. Extinctions occur at higher rates during times of ecological stress when populations and species are poorly adapted.

7 0
3 years ago
Read 2 more answers
Which ocean has the greatest average depth
Nadusha1986 [10]
I believe it is the pacific ocean as that is the ocean where the Mariana Trench is located, and the Mariana Trench is the deepest place in the Oceans
8 0
3 years ago
Read 2 more answers
In what cellular function does the structure here play a role
PIT_PIT [208]

Answer:

BRAINLIEST PLZZZ

Explanation:

They play a major role in protein synthesis. They act as the powerhouse for the cell. They are involved in the separation of chromosomes during cell division.

7 0
3 years ago
Read 2 more answers
At a fair or circus, some performers and clowns take long balloons and fold them into animals and objects as toys for children.
mixer [17]

The statement which explains why a balloon can fold, twist, and bend without bursting is: B. The balloon is filled with air, which is a mix of gases. Air is fluid and can move around the inside of the balloon as it is folded.

A balloon can be defined as a flexible rubber bag that can be inflated with a gas such as air.

Generally, air can be trapped in a balloon by sealing its neck tightly.

Hence, this makes it possible for performers and clowns to fold, twist, and bend an inflated balloon into animals and objects as toys for children.

A inflated balloon can be folded without it bursting simply because air is fluid and as such, it would move around the inside of the balloon as it is folded.

In conclusion, air, which is a mixture of gases would move around the inside of the balloon as it is folded because it is also a fluid.

Find more information: brainly.com/question/17203857

5 0
2 years ago
Other questions:
  • Which phrase does not describe one of the functions of proteins of the plasma membrane? initiating the replication of genetic ma
    7·1 answer
  • Most ______ immunizations must be re-administered on a regular basis.
    9·2 answers
  • The ______ system works with the circulatory system to maintain the correct water balance in cells.
    11·2 answers
  • A function of carbohydrates in the diet is to: enable chemical reactions. promote growth and repair of tissues. supply energy. m
    13·1 answer
  • Which of the following is one observation that Charles Darwin made regarding variation
    11·1 answer
  • Which membranous organelle is found next to the nucleus and is covered in ribosomes?
    15·2 answers
  • Pls help I AM ON A TIMERR PLEASE
    12·2 answers
  • Identify the organ of the digestive system.
    11·2 answers
  • Nitrogen fixation is one-way nitrogen moves through ecosystems. Why is nitrogen fixation necessary for ecosystems?
    12·2 answers
  • Which structure of human hands makes them especially useful for gripping things?
    6·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!