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Svetllana [295]
3 years ago
10

Why is it necessary to utilize probes for labeling particular dna sequences? how is this process accomplished?

Biology
1 answer:
Lana71 [14]3 years ago
7 0
importance of a probe
in hybridization process, molecular biologist label a short fragment of DNA or RNA, usually 10-1000 bases long tagged with a radioactive or fluorescent molecules. this fragment is usually referred to as hybridization probe.probe  important both in southern and northern blotting processed because it is  used to identify a certain sequence of DNA or RNA in a pool several sequences that have been obtained using nuclease.

process of labeling DNA sequence with a probe
after the DNA strand has been cut into several fragments using enzyme DNA nuclease, the fragments are separated using gel electrophoresis and then denatured using hcl to obtain strands. the single strand are then transferred to nylon membrane that has positive charge. the next step is where probes come in. to detect the DNA sequence of interest the nylon membrane is treated with solution containing tagged probes. since the probes have complementary sequence to the target sequence, it bind to that DNA sequence and detected later using X-ray film.     
  


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which one of the plants pictured below was given water mixed with salt, and which one was given pure water? Explain how you know
ludmilkaskok [199]

The plant on the right must have been the one given a saltwater mixture while the one on the left must have been given pure water.

<h3>Osmosis</h3>

Looking at the attached image closely, one would see that the plant on the right has withered leaves while the leaves of the plant on the left seem to be flourishing.

The plant on the right must have been the one fed with water mixed with salt and here is the reason. Adding water mixed with salt to the soil on which the plant grows will decrease the water potential of the soil relative to the water potential of the plant's root. Thus, water will move from the plant into the soil through the root by osmosis. This will cause the various cells of the plant to become flaccid, and hence, the withered leaves.

On the contrary, the plant on the left must have been fed with pure water. Feeding the soil with pure water will increase the water potential of the soil relative to the water potential of the plant's root. Thus, water will move from the soil into the plant through the root by osmosis. This will cause the various cells of the plant to become turgid, and hence, the flourishing leaves.

More on osmosis can befound here: brainly.com/question/21395644

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3 0
2 years ago
Explain how a dog meets all of the characteristics of life.
stealth61 [152]

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A dog meets all the characerisitcs of life in the following ways - they maintain homeostatis, are made of cells, breathe and have a metabolism, are able to reproduce, pass their traits off to their offsp they grow and change, and and respond to their environment.

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8 0
2 years ago
explain how the information in this diagram indicates that an oxygen atom could bond covalently to two hydrogen atoms to form wa
zhannawk [14.2K]

Answer:

because when oxygen atom bonds with two hydrogen atoms they form water

Explanation:

A water molecule is formed by covalent bonds between an oxygen atom and two hydrogen atoms. The bond between hydrogen and oxygen involves unequal sharing of electron – it is a polar covalent bond. This is because the nucleus of the oxygen atom is more attractive to electrons than the nuclei of the hydrogen atom.

5 0
3 years ago
What happened to the California Condors?
expeople1 [14]

Answer:

B

Explanation:

California Condors have been in decline about as long as European Settlements began to spread across North America. These birds have been on the U.S endangered species list since 1967 and were near extinction when their captive-breeding program began. California Condors also mature and reproduce slowly.

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3 years ago
Which is an example of asexual reproduction in plants
Levart [38]
An example would be potatoes because they reproduce asexually through their roots.

4 0
3 years ago
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