Answer: For detection and confirmation of HIV antibodies in blood samples.
Explanation: As the name implies ELISA( Enzyme-linked immunosorbent assay) is the first test widely used for determining the presence of HIV in a person's serum because of its high sensitivity. In an ELISA, a person's serum is diluted 400 times and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" (an antibody that binds to other antibodies) is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme.
Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. Unknown samples that generate a stronger signal than the known or control sample or are called "positive" while those that generate weaker signal are "negative".
Answer:
LAIV should not be administered to: Children aged <2 years of age.
Explanation:
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The senses of warmth, cold, pain, and touch (pressure) located in the skin.Skin sensations are the source of relatively simple experiences such as itching and tingling, pain due to injury, and feelings of hot and cold. Though they often play a crucial role in our lives, we depend far less on them than on sight and hearing for our knowledge of the external world. However, one of these senses, the sense of touch, is more closely related to perception than the others, since we can often identify objects by the way they feel.
It would be more difficult for a researcher to establish rapport with an informant they have not met.
Informants are very important in research because they help the researcher to find out things that would have been otherwise difficult to find.
Researchers generally prefer to meet their informants in person because:
- they can better read non-verbal cues such as hand gestures
- there is a chance for a better connection with the informant
- easier to establish credibility
All of these ensure that the researcher is able to establish rapport easier with the informant so meeting in person is better.
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It would not benefit, or make little cure. Also, it would not make a change in how you feel. Lastly, it would look biased from the package.
