Answer: B. Melting temperatures of primer should be between 55-80 degree Celsius.
Explanation:
Bacause the melting temperature controls the binding of the primers to your template DNA. At melting temperature 50% of the primer molecules are bound to their corresponding target sequence. If the difference in melting temperature between the two primers is too high, it might be difficult to find experimental conditions where both primers can bind to their target.
Thymine
The two strands are held together by hydrogen bonds between the bases, with adenine forming a base pair with thymine, and cytosine forming a base pair with guanine.
<span>If one determines that wolves are experiencing a reduction in their population that can be attributed to a virus the coyotes carry that is more harmful in wolves. this is an example of apparent competition.</span>
<span>Domain: Bacteria
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Enterobacteriales
Family: Enterobacteriaceae
Genus: Escherichia
Species: Escherichia coli (E. coli)</span>
I believe the answer is sunlight