Question

Assume you inoculated 100 cells, with a generation time of 20 minutes, into 100 ml of nutrient broth. You then inoculated 100 cells of the same species into 1000 ml of nutrient broth. After incubation for 3 hours, you can reasonably expect to have:

Answer 1

Answer:

the same number of cells in both.

Explanation:

Nutrient broth is a general purpose medium that can be used to cultivate different variety of both fastidious and non-fastidious microorganisms.

Nutrient broth is known to contain or compose of simple peptone and a beef extract.

It is known to be used for bacteria that will not form colonies  on a solid surface.

Nutrient broth can be prepared through this following steps

* Add 13g of nutrient broth powder  in 1L of distilled water.

* Mix and dissolve them completely.

* Pour them into the final containers (eg. conical flask)

* Sterilize by autoclaving at 121°C for 15 minutes.

In this case, since the 100 ml  and 1000 ml is prepared using the same method, it is expected to have the same number of cells in the same volume of the broth.

Answer 2

Hello. You forgot to enter the answer options. The options are:

"A) the same number of cells in both.

B) more cells in the 100 ml.

C) more cells in the 200 ml.

D) The answer cannot be determined based on the information provided"

Answer:

A) the same number of cells in both.

Explanation:

You inoculated the same amount of cells of the same species in nutrient broths of different amounts. It must be kept in mind that as the cells are of the same species, they have the same reproductive capacity, they will reproduce in the same ways.

Regarding the difference in volume of the nutrient broths, the broth with the lowest volume (100mL) presents the nutrients in a more concentrated way allowing the cells to have access to these nutrients faster and be able to reproduce more quickly. The nutrient broth with the largest volume (1000mL) will have the nutrients in a more diluted form, causing the cells to reproduce more slowly, but with the same reproductive capacity as the cells in the other broth. These factors will allow us to observe the same number of cells in both broths after 3 hours.