What's are those first 3?
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You should mix 4µl of DNA sample and complete it with 96µl (100-4) of water.
You can figure this out by using C1.V1 = C2.V2
C1 = 250 <span>ng/μl (Sample Concentration)
</span>V1 = ? (the necessary volume to take from the DNA sample)
C2 = 10 <span>ng/μl (PCR concentration)
</span>V2 = 100 µl (total volume)
V1 =
= 
= 4µl
For the water: 100µl (total volume) = 4µl + Water
Water volume = 96µl
You can figure this out by using C1.V1 = C2.V2
C1 = 250 <span>ng/μl (Sample Concentration)
</span>V1 = ? (the necessary volume to take from the DNA sample)
C2 = 10 <span>ng/μl (PCR concentration)
</span>V2 = 100 µl (total volume)
V1 =
For the water: 100µl (total volume) = 4µl + Water
Water volume = 96µl