Answer:
PCR is known as polymerase chain reaction used to amplify DNA sequence of our interest into multiple copies. This technique is been commonly used by researchers to study in depth about the gene of interest during their research work.
Explanation:
PCR:- It is known as polymerase chain reaction, used to amplify DNA sequence of our interest into multiple copies mainlty to millions or trillions
<u>Components</u>
Phusion HF buffer:- This buffer Create optimal reaction conditions for high fidelity amplification of DNA
dNTPs:- They are the building blocks in the synthesis of new copies of DNA.There are four dNTPs used in the amplification process that is dATP, dGTP, dCTP, dTTP. these building blocks are added in equal proportion during the PCR reaction
Phusion DNA polymerase:- This is the enzyme used in DNA amplification, it is generally a fusion of DNA binding domain to a portion of pyrococcus like proof reading polymerase. This enzyme is tolerant to various inhibitors, allowing strong amplification of DNA of interest with minimal optimization
pCD122:- Plasmid that serves as a template DNA for the amplification of desired DNA sequence of our interest.
sense primer CD474:- Sense primer is also known a reverse primer, it attaches to the stop codon of the complementary strand of DNA
antisense primer CD475:- This primer is also known as forward primer, it attaches to the start codon of the template DNA
All these components is added in such a way that the total mixture should have a reaction volume of 30.0μl
No species exists in a vacuum; every form of life on Earth interacts over time with other organisms, as well as with its physical environment. For that reason, the evolution of one species influences the evolution of species with which it coexists by changing the natural selection pressures those species face.
Answer: The chemical symbol always begins with a capital letter.
Explanation: If you look at the periodic table, there is no symbol which starts with a small letter
Answer:
D
Explanation:
you can't take one type of cell and create a brand new one. This gets rid of C
the only cell that increases in size is fat cells, this gets rid of A
Mitosis is for all cells, except sex cells. Daughter cells relate more to sex cells than other cells. Sex cells go through meiosis to create daughter cells. This also gets copies DNA. This gets rid of B
Basically: Mitosis is how we make new cells from the original cell (Think Zygote cell)
The addition or removal of heat changes the temperature of an object. The temperature can cool down or it can warm up.