Answer:
Since high ethanol is a major stress during ethanol fermentation, ethanol-tolerant yeast strains are highly desirable for ethanol production on an industrial scale. A technology called global transcriptional machinery engineering (gTME), which exploits a mutant SPT15 library that encodes the TATA-binding protein of Saccharomyces cerevisiae (Alper et al., 2006; Science 314: 1565-1568), appears to be a powerful tool. to create ethanol tolerant strains. However, the ability of the strains created to tolerate high ethanol content in rich media remains to be demonstrated. In this study, a similar strategy was used to obtain five strains with higher ethanol tolerance (ETS1-5) of S. cerevisiae. When comparing the global transcriptional profiles of two selected strains ETS2 and ETS3 with that of the control, 42 genes that were commonly regulated with a double change were identified. Of the 34 deletion mutants available in an inactivated gene library, 18 were sensitive to ethanol, suggesting that these genes were closely associated with tolerance to ethanol.
Explanation:
Eight of them were novel and most were functionally unknown. To establish a basis for future industrial applications, the iETS2 and iETS3 strains were created by integrating the SPT15 mutant alleles of ETS2 and ETS3 into the chromosomes, which also exhibited increased tolerance to ethanol and survival after ethanol shock in a rich medium. Fermentation with 20% glucose for 24 h in a bioreactor revealed that iETS2 and iETS3 grew better and produced approximately 25% more ethanol than a control strain. The performance and productivity of ethanol also improved substantially: 0.31 g / g and 2.6 g / L / h, respectively, for the control and 0.39 g / g and 3.2 g / L / h, respectively, for iETS2 and iETS3.
Therefore, our study demonstrates the utility of gTME in generating strains with increased tolerance to ethanol that resulted in increased ethanol production. Strains with increased tolerance to other stresses such as heat, fermentation inhibitors, osmotic pressure, etc., can be further created using gTME.
Answer:
Nitrogen cycle works through various stages like, nitrogen fixation, nitrification, assimilation, ammonification, denitrification etc. It is a building block for protein and DNA
Explanation:
Nitrogen is an element abundantly found in the atmosphere, also its building block for proteins as well as nucleic acid i.e. DNA formation. In nitrogen cycle , the nitrogen is being prepared from inert nitrogen. The nitrogen cycle has several processes like nitrogen fixation, in this the inert nitrogen is being converted to organic nitrogen with the help of certain micro-organism.
Nitrification, plants cannot absorb directly nitrogen so bacteria help the plants to convert the nitrogen into ammonia form in this stage. Assimilation, another stage in which plants starts absorbing various forms of nitrogen from the soil.i.e. nitrate, nitrite and ammonium. Ammonification, here plants and animals have nitrogen in there body after death various microbes help in decomposition in this stage. Denitrification, in this stage the return back of nitrogen takes place.
Answer:
The testes are two oval-shaped male reproductive glands that produce sperm and the hormone testosterone.
Shaping. Each step in the learning process is called an approximation. An animal may be reinforced for each successive approximation toward the final goal of the desired trained behavior. Animals learn complex behaviors through shaping.
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