Answer: silent mutation: a nucleotide base in a codon is replaced with a different base BUT the resulting amino acid isn’t affected.
Missense mutation: similar to a silent mutation, the only difference is that the switch of the nucleotide DOES result in a DIFFERENT amino acid
Nonsense mutation: a nucleotide base is changed, but that change results in a premature stop of translation
(Remember that after transcription, you are left with a strand of mRNA that Is then translated into a protein. The mRNA is read in increments of three nucleotide bases (A,U,G, or C) which is called a codon. That codon makes a single amino acid, and a strand of amino acids makes a protein)
Eutrophication of water bodies like lake, pond, shallow stretches of river, etc. is the phenomenon where excess growth of vegetation on the surface of the water takes place. This excess growth results in the clouding of the water, depletion of dissolved oxygen in water, and the death of aquatic organisms. The primary reason for the cause of eutrophication is the presence of nutrients in excess amounts in the water. The nutrients B. come from fertilizer and sewage runoff.
Earth's atmosphere helps maintain a constant temperature.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
The possibility to answer is C.
