The total solution formed after mixing the components will be
20 μl + 20 μl + 40 μl + 120 μl = 200 μl
The smallest size tube that will hold the entire reaction is:
Firstly convert 200μl to ml
1000 μl = 1 ml
200 μl = X
X = 200 x 1/1000
X = 0.2 ml
The smallest size tube (from the options provided in the question) is 0.25 ml.
The pipetting device that could be used to accurately measure all the needed volumes must be able to measure 20 μl, 40 μl and 120 μl.
The best pipette to accurately take these measurements individually is the P200 micropipette because it has an accurate/useful range of between 20 μl - 200 μl
the orientation of Earth's tilt with respect to the sun – our source of light and warmth – does change as we orbit the sun. In other words, the Northern Hemisphere is oriented toward the sun for half of the year and away from the sun for the other half.
The PUPIL is the hole in the MIDDLE of the IRIS of the eye, through which light passes to be focused on the RETINA.
The IRIS is the contractile membrane perforated by the pupil, which adjusts to control the amount of light reaching the retina, and which forms the colored portion of the eye
ACCOMMODATION is the change in the adjustment of the eye lens to help focus light ray.
RETINA helps to receive light rays that the lens has focused. It contains two cells: rods and cones
Fovea Centralis is at the center of the retina responsible for sharp and accurate vision, also it is where cones cells cluster.
