Answer:
Explanation:
The total solution formed after mixing the components will be
20 μl + 20 μl + 40 μl + 120 μl = 200 μl
The smallest size tube that will hold the entire reaction is:
Firstly convert 200μl to ml
1000 μl = 1 ml
200 μl = X
X = 200 x 1/1000
X = 0.2 ml
The smallest size tube (from the options provided in the question) is 0.25 ml.
The pipetting device that could be used to accurately measure all the needed volumes must be able to measure 20 μl, 40 μl and 120 μl.
The best pipette to accurately take these measurements individually is the P200 micropipette because it has an accurate/useful range of between 20 μl - 200 μl
