This affected slaves in a negative way because since the fugitive slaves would be returned to their owner, the slaves would later suffer punishment, beatings, and even death due to them escaping their master. It was a terrible agreement that no state could stop a fugitive slave from being returned to his/her owner.
A=2 B=red blood cells rely on catalase, catalase decomposes hydrogen peroxide C=The effect of replacing his is that the hydrophobic amino acid would not do well with water D= the hydrophobic amino acid is like oil and water and wouldn’t do well decomposing hydrogen peroxide