Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
Gene flow
Explanation:
Evolution is the changes seen in the growth, development, features of a certain group of species during the cause of time. Evolution causes changes in the gene frequency of species from one generation to the next generation.
There are 5 important mechanisms of evolution and they are: Gene flow, mutation, genetic drift, natural selection and non random mating.
Gene flow as a mechanism of evolution is the exchange or transfer of genes between a group of species as they move from one place to another. Gene flow can either increase the rate of evolution or decrease it.
The genotype, I believe would be the dominant long wings (W). Hope this helps!
Answer:
they have a 25% chance of having a son with hemophilia and a 25% chance of having a daughter with it.
Explanation:
Hemophilia is an linked gene so when you create a punnet square those are the result you should receive.
Yes, you are doing this correct.
