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When a hairpin loop forms in the nascent mRNA: The hairpin will destabilize the interaction and possibly lead to transcriptional termination.
Transcription in prokaryotes like E. coli is terminated either by a rho-dependent process or a rho-independent process. Intrinsic termination is controlled by the specific sequences of RNA .
When the termination process starts, the transcribed mRNA forms a stable secondary structural hairpin loop, also known as a stem-loop. Several uracil nucleotides follow this RNA hairpin. The uracil and adenine connections are exceedingly weak. NusA, a protein attached to RNA polymerase, attaches to the stem-loop structure so firmly that it momentarily stalls the polymerase.
The polymerase is pausing at the same time that the poly-uracil sequence is being transcribed. The RNA-DNA duplex can unwind and separate from the RNA polymerase because the weak adenine-uracil interactions reduce the energy of destabilization for the RNA-DNA duplex. Overall, transcription is terminated by the modified RNA structure.
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Any mutations in DNA are forever, since DNA is the master blueprint. All mutations will be passed onto new cells through DNA replication, and all RNA made from that DNA will also have the mutation. If the mutation occurs in sperm or egg cells, those mutations will also be passed on to the organism's offspring.
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