Site-specific recombination systems all of the choices are correct i.e.
A. do not depend on extensive nucleotide sequence homology.
B. depend on enzymes that are often specific for sequences within the host.
C. are features of some viruses.
- An exchange between two specified sequences (target sites), either on the same DNA molecule or on two separate DNA molecules, is known as site-specific recombination.
- DNA sequences may be integrated, excised, or inverted as a result of the exchange.
- A site-specific recombinase that can work by itself or with the aid of additional components or enzymes shapes the DNA target during recombination.
- The recombinase is chemically bonded to the ends of the intermediate DNA after DNA breakage at the recombination site; when this process is reversed, the intermediate DNA is resealed to form the recombinant and the recombinase is released.
- During this recombination process, neither replication nor repair are necessary.
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