If the a decomposer were absent from the forest, everything would just pile up onto one another and never be returned to the Earth.
Metaphase I believe that is the answer to your question
Answer: After 3 rounds, 8 segments having 2 original strands on different segments will be present.
Explanation:
The polymerase chain reaction selectively and repeatedly copy the double stranded DNA (dsDNA) in the PCR machine.
1st round:
Single piece of double stranded DNA (dsDNA) unwinds in two seperate strands, before complementary nucleotides are synthesized to establish double helix.
2nd round:
The two dsDNA yields 4 segments before complementary nucleotides are synthesized
3rd round:
The four dsDNA yields 8 segments before complementary nucleotides are synthesized.
Therefore, at the end of 3 rounds, the two original strands will be present on the 8 different segments of copied DNA.
Insertion mutation takes the T out and replaces it with an A.