No, even after several days of incubation, you shouldn't detect any symptoms of microbial growth in a sterile medium.
<h3>What is the microbial growth in the sterile medium?</h3>
The deliberate introduction of germs into a sterile growing medium is known as immunization. When there are no living creatures present, a substance is sterile; undesirable bacteria are said to be contaminated. The use of aseptic procedures helps keep growing media from being contaminated. Reduce the amount of time that cultures and growth media are exposed to the outside world. Clean the work area both before and after each use. Avoid breathing or touching the stock cultures or sterile culture media. Before used, loops, needles, pipes, and other items should be sanitized. The tube caps should be held in your hand while inoculating and not placed on the table while using tubes.
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