Answer:
The correct answer is A. The cartilaginous structure between the "throat" and the trachea is the larynx.
Explanation:
The larynx is a tubular organ. The larynx wall is composed of 9 pieces of cartilage. Three are odd (thyroid cartilage, epiglottis and cricoid cartilage), and three pairs (arytenoid, cuneiform and corniculate cartilage). In addition, it communicates the pharynx with the trachea and is in front of it.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
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