Scientists have engineered bacteria to produce human proteins, such as human growth hormone (hGH) used to help treat dwarfism. S
uppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene.
Bacteria do not possess the tendency to withdraw sequences of introns from a gene, thus, if the gene for the human growth hormone were transcribed, it would translate into a non-functional protein.
When the expression of a gene takes place in eukaryotes, the budding mRNA comprising introns are removed consequently at the time of post-translational processing to produce mature mRNA. Also, the human growth hormone is produced by the pituitary gland in the form of a pre-hormone comprising a leader peptide of about 20 amino acids in length, which need to get removed post-translationally to produce a mature functional protein.
Bacteria do not possess the biochemical machinery either to effectively withdraw the leader peptide after translation or to splice out the introns. Thus, when an unchanged human growth hormone is cloned, the bacteria cannot produce the functional human growth hormone.
Prokaryotic cells have a cell wall surrounding their cell membrane .The Prokaryotic cells don't have a well defined nucleus .The genetic material of the cell is basically naked and not enveloped by the Nuclear membrane .
Eukaryotic cells don't have a cell wall surrounding their cell membrane. The Eukaryotic cells have a well organised nucleus .The genetic material of the cell is surrounded by nuclear membrane or nuclear envelope.
Both Eukaryotic and prokaryotic cells have DNA.Both have a plasma membrane or cell membrane.
