Answer:
The correct answer will be option-A.
Explanation:
Recombineering is a molecular technique based on the recombination mechanism of DNA which is also known as recombination-mediated genetic engineering.
This method is used mainly in bacterial genetics to generate conditional mouse knockout target vector and modifying DNA in Bacterial artificial chromosome.
Since it does not use older methods like restriction enzymes and DNA ligases to combine DNA, therefore, option-A is the correct answer.
Answer:
gDNA = "genomic DNA" and cDNA = "complementary DNA." cDNA is classically associated with being reverse transcribed either from all extracted RNA from a tissue or cell (total RNA) including (in eukaryotes) pre-mRNA, ribosomal RNA, tRNA, snoRNA, miRNA and mRNA, etc.) while cDNA obtained only from reverse transcription of the mRNA (expressed eukaryotic cytosolic mRNA) fraction (e.g., by poly[dT]n and random priming) is complementary DNA (cDNA) made from what is called the "transcriptome." Eukaryotes have introns and exons in the gDNA, while prokaryotes do not. So eukaryotic cDNA reverse transcribed from mRNA lacks introns. Prokaryotic-derived cDNA is always complementary to prokaryotic RNA and gDNA (so is always necessary to have a good DNase treatment prior to gene expression analysis by e.g., qPCR for prokaryotic transcriptome work)...
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❖ I believe it is considered as a macromolecule.
~ ʜᴏᴘᴇ ᴛʜɪꜱ ʜᴇʟᴘꜱ! :) ♡
~ ᴄʟᴏᴜᴛᴀɴꜱᴡᴇʀꜱ
Answer:
<em><u>mark</u></em><em><u> </u></em><em><u>me</u></em><em><u> </u></em><em><u>brainliest</u></em>
Explanation:
<h2>Enzymes work consistently until they are dissolved, or become denatured. When enzymes denature, they are no longer active and cannot function.</h2>
<span>helicase<span>DNA helicase is the enzyme that unwinds the DNA double helix by breaking the hydrogen bonds down the center of the strand. It begins at a site called the origin of replication, and it creates a replication fork by separating the two sides of the parental DNA.<em /></span></span>