What is the basis of Gram staining results?
1 answer:
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Discovery
In 1928 it was discovered by Frederick Griffith in an experiment generally known as transformation.
Experiment
In his experiment he considered two strains of <em>streptococus pneumonia,</em> one was R-type which was non-virulent and cause no disease in mice, other was virulent and S-type which cause disease and at last death of mice.
This experiment was comprised of four steps which are as follow:
Step 1:
First he injected living strain of S into mice, after sometime mouce died.
Step 2:
He injected living strain of R into mice, the mice alive as he did not got any disease.
Step 3:
He injected heat killed strain of S into mice and mice remain alive.
Step 4:
He mixed living R strain with heat killed S strain and then inject into mice. As a result the mice died.
Conclusion:
It was found that genetic material from heat killed S stain were transferred to living R (non-virulent) strain, as a result R become virulent and cause the death of mice.
In 1928 it was discovered by Frederick Griffith in an experiment generally known as transformation.
Experiment
In his experiment he considered two strains of <em>streptococus pneumonia,</em> one was R-type which was non-virulent and cause no disease in mice, other was virulent and S-type which cause disease and at last death of mice.
This experiment was comprised of four steps which are as follow:
Step 1:
First he injected living strain of S into mice, after sometime mouce died.
Step 2:
He injected living strain of R into mice, the mice alive as he did not got any disease.
Step 3:
He injected heat killed strain of S into mice and mice remain alive.
Step 4:
He mixed living R strain with heat killed S strain and then inject into mice. As a result the mice died.
Conclusion:
It was found that genetic material from heat killed S stain were transferred to living R (non-virulent) strain, as a result R become virulent and cause the death of mice.