I believe your answer would be B forgive me if im wrong
The red-legged frog breeds in ephemeral ponds from January through March. Its relative, the bullfrog, breeds in permanent ponds from late March through May. There are two pre-mating isolation mechanism at play here,
1. Ecological isolation: The habitats are different, hence the individuals of both the species do not meet. Ephemeral ponds are temporary ponds that develop during rainy days, while the permanent ponds are full of water throughout the year.
2. Temporal isolation: The time of mating is different for both the species to avoid contact between the individuals of closely related species. Red-legged frogs mate in January to March slot and the bullfrog in the March to May slot.
Answer:( answer for number one) an essential nutrient for the production of amino acids, proteins, nucleic acids. ( number 2 ) for the growth, maintenance, and repair of all tissues and cells, and for the production of the genetic building blocks, DNA and RNA
It would be temp if my teacher explained it right. hope this helps