Answer: radiation i think
RNA contains cytosine and uracil as pyrimidine. DNA has cytosine and thymine.
Answer: To eliminate bacteria that failed to take in the plasmid.
Explanation:
Research shows that plasmid DNA is only taken in by small percentage of bacteria in a large population.
Therefore, the need of growing transformed bacteria in the presence of ampicillin is very important. The main reason of this process is to kill off or eliminate bacteria that have failed to take in the plasmid, due to their possession of recombinant DNA plasmid pARA-R (an ampicillin resistance gene), that allows them to survive.
However, after completion of the aforementioned procedure, the bacteria that have been transformed will be able to take in the plasmid.
