Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Answer:
During cellular respiration, <em>glucose</em> and oxygen are converted into carbon dioxide and water.
Explanation:
The daughter cells produced from Meiosis are genetically different from each other due to the process of Crossing Over in the stage of Prophase I.
In Mitosis, there is no Crossing Over involved, since it is intended to help the body in growth and repair, not in reproduction.
Genetic Variation is important for reproduction so that not all offspring look exactly like each other or so that they do not look exactly like their parents.
Option D order includes fewest organisms
