<span>B.The existence of vestigial structures
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Vestigial structures have been used for long by evolutionists to support the theory of evolution. These organs are believed to have been in use in the past lives but as the species adapts to new forms of life and lifestyle,the vestige organs becomes obsolete,but are not removed by the body. An example of vestige organs in human being are the appendix,tail bone,and mammalian grands in men.
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so, an example of something that might not be a substrate is the animal itself.
explanation
substrate is the surface of where living animals, live.
sorry if this doesnt help.
HAPPY ALMOST HALLOWEEN
The bladder is the muscular organ that holds urine