The plasmid must express a gene for ampicillin resistance (the protein product of the <em>bla</em> gene codes for beta-lactamase, the protein that breaks down ampicillin). The colonies on the ampicillin plate are antibiotic resistant. This means that they have taken up the transformed plasmids expressing both the <em>bla</em> gene and the GFP gene.
Explanation:
The transformation involved the genetic modification of a plasmid to incorporate the gene encoding the green fluorescent protein (GFP) from jelly fish. GFP makes cells glow under UV light.
In genetic engineering, scientists use antibiotic resistance as markers to indicate cells that have been transformed. By incorporating an antibiotic resistance gene such as <em>bla</em> into the vector (plasmid) and then growing the cells in antibiotic media, scientists determine which colonies have taken up the plasmid. Therefore, if the cells survive, this means that they contain the plasmid with antibiotic resistance gene as well as the GFP gene.
Animals are repeatedly eating the plastic, getting stuck in the plastic, and the plastic polluting their homes. There is also a place in the Pacific called "The Great Pacific Garbage Patch" and it is roughly 1.6 Million square Km.
Each time you exhale, you are releasing carbon dioxide gas (CO2) into the atmosphere. Animals and plants get rid of carbon dioxide gas through a process called respiration. Carbon moves from fossil fuels to the atmosphere when fuels are burned.
