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LekaFEV [45]
4 years ago
7

If the genetically transformed cells have acquired the ability to live in the presence of the antibiotic ampicillin, then what m

ight be inferred about the other genes on the plasmid that you used in your transformation procedure?
Biology
1 answer:
V125BC [204]4 years ago
5 0

Answer:

The plasmid must express a gene for ampicillin resistance (the protein product of  the <em>bla</em> gene codes for beta-lactamase, the protein that breaks down ampicillin). The colonies on the ampicillin plate are antibiotic resistant. This means that they have taken up the transformed plasmids expressing both the <em>bla</em> gene and the GFP gene.

Explanation:

The transformation involved the genetic modification of a plasmid to incorporate the gene encoding the green fluorescent protein (GFP) from jelly fish. GFP makes cells glow under UV light.

In genetic engineering, scientists use antibiotic resistance as markers to indicate cells that have been transformed. By incorporating an antibiotic resistance gene such as <em>bla</em> into the vector (plasmid) and then growing the cells in antibiotic media, scientists determine which colonies have taken up the plasmid. Therefore, if the cells survive, this means that they contain the plasmid with antibiotic resistance gene as well as the GFP gene.

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Answer:

All of the above

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Which of the following statements about prokaryotic cells is correct?
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Answer:

A mutation during protein synthesis may not affect the protein since the mutation causes a new code for the same amino acid, the protein will still function normally.

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In protein synthesis, when a mutation alters the codon, changing one nucleotide for another, it is possible that the mutation does not alter the amino acid and the protein functions correctly. This is because an amino acid can be encoded by several codons, as occurs with leucine, which is encoded by CUU, CUC, CUA and CUG.

<em>     The other options are not correct because one mutation does not give better amino acids or more powerful proteins.</em>

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