Endotherms, such as birds and mammals, use metabolic heat to maintain a stable internal temperature, often one different from the environment. Ectotherms, like lizards and snakes, do not use metabolic heat to maintain their body temperature but take on the temperature of the environment.
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Water is least dense at 0°C (freezing point)
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When a protein is denatured, secondary and tertiary structures are altered but the peptide bonds of the primary structure between the amino acids are left intact. Since all structural levels of the protein determine its function, the protein can no longer perform its function once it has been denatured.A protein becomes denatured when its normal shape gets deformed because some of the hydrogen bonds are broken. Weak hydrogen bonds break when too much heat is applied or when they are exposed to an acid (like citric acid from lemon juice).