Question

You are interested in the detailed effects of cytochalasin D on microfilaments over time. Based on what you know about the molecular mechanism of action of cytochalasins, describe what happens to MFs in cells treated with the drug. In particular, explain why existing actin polymers eventually depolymerize.

Answer 1

Answer:

Basically  Cytochalasin D inhibits   the rate of actin polymerization and how  actin filaments  interacts with its barbed end in solution.

It does this by inhibiting the addition of more actin monomers to the 'barbed' or 'unsealed'  end of the filaments meant for rapid addition of monomers. On binding,it blocked or capped the barbed end  of the actin filaments inhibiting,monomers addition and  polymerization.

Thus  the failure of addition  of more monomers  inhibits polymerization and prevent elongation of actin filaments. Generally,Cytochalasin inhibits polymerisation at   low concentration .Thus at low concentration of 2uM  its inhibits actin  polymerization by 90%.

Answer 2

Answer:

Actin depolymerizing factor binds F-actin cooperatively and induces a conformational change in F-actin that causes it to twist further and become more destabilized. This twisting causes severing of the bond between actin monomers, depolymerizing the filament.

Explanation:

Actin filament depolymerization ensures the turnover of actin filaments within these structures and maintains a pool of actin monomers that permits the continual restructuring and growth of the actin cytoskeleton. ADF/cofilin influences actin filament turnover.