Sterile powdered agar with nutrients can be mixed with water, heated and then poured into empty petri plates or ready-to-use dishes can be purchased. The undigestible agar is a gelatin-like substance with a semi solid surface on which the bacteria can grow while they consume the added nutrients (like sheep's blood).
Different cell types were found in the tumor and murine liver using XYZeq.
Tumor:
- The extraordinary variability of cell kinds and states in tissues has been shown by single-cell RNA sequencing (scRNA-seq), but it does not offer information about the spatial organization of cells. We created XYZeq, a technique that embeds spatial metadata into scRNA-seq libraries, to better understand how individual cells behave within an anatomical area. Tens of thousands of cells' spatially barcoded transcriptomes were collected using XYZeq to profile mouse tumor types. In tumor-associated mesenchymal stem cells, analyses of these data revealed the geographic distribution of several cell types and a cell migration-associated transcriptome program (MSCs). The MSCs' localized expression of tumor suppressor genes, which varies with proximity to the tumor core, is another finding. We show that the spatial localization of the transcriptome and XYZeq can be mapped.
- A tumor is a mass of tissue that forms inappropriately when cells either enlarge and divide more frequently than they ought to or do not die as expected. Tumors without cancer may be benign or malignant (cancer).
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